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KMID : 0380620080400060603
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Korean Journal of Food Science and Technology
2008 Volume.40 No. 6 p.603 ~ p.610
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Quantification of Protein and Amylose Contents by Near Infrared Reflectance Spectroscopy inAroma Rice
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Kim Jeong-Soon
Song Mi-Hee
Choi Jae-Eul
Lee Hee-Bong
Ahn Sang-Nag
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Abstract
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The principal objective of current study was to evaluate the potential of near infrared reflectance spectroscopy(NIRS) as a non-destructive method for the prediction of the amylose and protein contents of un-hulled and brown ricein broad-based calibration models. The average amylose and protein content of 75 rice accessions were 20.3% and 7.1%,respectively. Additionally, the range of amylose and protein content were 16.6-24.5% and 3.8-9.3%, respectively. In total,79 rice germplasms representing a wide range of chemical characteristics, variable physical properties, and origins werescanned via NIRS for calibration and validation equations. The un-hulled and brown rice samples evidenced distinctlydifferent patterns in a wavelength range from 1,440 nm to 2,400nm in the original NIR spectra. The optimal performancecalibration model could be obtained by MPLS (modified partial least squares) using the first derivative method (1:4:4:1)for un-hulled rice and the second derivative method (2:4:4:1) for brown rice. The correlation coefficients (r2) and standarderror of calibration (SEC) of protein and amylose contents for the un-hulled rice were 0.86, 2.48, and 0.84, 1.13,respectively. The r2 and SEC of protein and amylose content for brown rice were 0.95, 1.09 and 0.94, 0.42, respectively.The results of this study suggest that the NIRS technique could be utilized as a routine procedure for the quantificationof protein and amylose contents in large accessions of un-hulled rice germplasms.
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KEYWORD
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NIR spectrum, aroma rice, amylose, germplasm evaluation, MPLS
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